High intensity interval training (HIIT) boosts the amount of proteins in skeletal muscle that are essential for energy metabolism and muscle contraction, and chemically alters key metabolic proteins in men, according to a study by the University of Copenhagen published in eLife.
The results may explain the beneficial effects of HIIT on metabolism and pave the way for additional studies exploring how exercise impacts these processes, according to the researchers.
“Exercising has many beneficial effects that can help prevent and treat metabolic diseases, and this is likely the result of changes in energy use by skeletal muscles,” Morten Hostrup, associate professor at the Department of Nutrition, Exercise, and Sports at the University of Copenhagen, Denmark, and first and co-corresponding author of the study, said in a media release. “We wanted to understand how exercise alters the muscles’ protein content and how it regulates the activity of these proteins through a chemical reaction called acetylation.”
Acetylation occurs when a member of the small molecule group, acetyl, combines with other molecules and can affect the behavior of proteins.
For their study, the researchers recruited eight healthy, untrained male volunteers to complete five weeks of high-intensity cycling training. The men worked out three times per week, finishing four minutes of cycling at a target rate of more than 90 percent of their maximum heart rate followed by a two-minute rest. They repeated this pattern four to five times per workout.
Using a technique called mass spectrometry, the team analyzed changes to the composition of 3,168 proteins in tissue samples collected from the participants’ thighs before the study and after they completed the training. They also examined changes relating to 1,263 lysine acetyl-sites on 464 acetylated proteins.
Their analysis showed an increase in the production of proteins used to build mitochondria, which produce energy in cells, and in proteins related to muscle contractions. The team also identified increased acetylation of mitochondrial proteins and enzymes that are involved in the production of cellular energy.
Additionally, they observed changes in the amount of proteins that reduce the skeletal muscle’s calcium sensitivity, which is essential for muscle contractions.
The results confirm some well-known changes to skeletal muscle proteins that occur after exercise, as well as identify new ones. For example, the reduced calcium sensitivity may explain why it can be harder for muscle contraction to occur after an athlete becomes fatigued.
The work also suggests that exercise-induced changes in the regulation of proteins through acetylation may contribute boosting metabolism.
“Using state-of-the-art proteomics technology, our study provides new information about how skeletal muscle adapts to exercise training, including the identification of novel exercise-regulated proteins and acetyl-sites,” said co-corresponding author Atul Deshmukh, who is associate professor at the Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen. “We hope our work will stimulate further research into how exercise helps improve metabolic health in humans.”